|
KMID : 0382619840040020413
|
|
Hanyang Journal of Medicine
1984 Volume.4 No. 2 p.413 ~ p.424
|
|
|
An Experimental Study for the Phagocytic and Digestive Activity of RES in Endotoxin Injected Rat
|
|
李寅貴/Lee, In Kyi
崔泰烈/金春元/Choi, Tae Yeal/Kim, Choon Won
|
|
|
Abstract
|
|
|
Endotxemia is frequently experienced dlinically, and it rapidly progresses toward death by shock. The endotoxin is a protein-lipopolysacchride complex, which mainly acts upon plasma coagulation factor, platelet, leukocytes and reticuloendothelial system. The liver and spleen are major target organs in endotoxemia.
To study the mechanism for the phagocytic and digestive action of reticuloendothelial system, mainly liver and spleen, in rats. An experimental groups were devided as follows; 1 ㎖ normal saline (GroupⅠ), Staphlococcus aureus 10℃FU 1 ㎖ (ATCC 25922, Group Ⅱ), E. coli endotoxin 0.3㎎/㎏(lipopolysacc Difco 026:B6, Group Ⅲ) and Staph. aureus 10℃FU/㎖ with E. `coli endotoxin 0.3㎎/㎏ (Group Ⅳ) were into tail vein, respectively. Studies included alkaline phosphatase and alanine aminotransferase in serum, viable colony count of blood, liver and spleen and the histopathogic observation of liver and spleen.
The obtained results are follows:
1. Alkaline Phasphatase and alanine aminotransferase are markedly increased in Group Ⅲ and Ⅳ.
2. The number of viable bacteria of blood is gradually decreased according to time in group Ⅱ and Ⅳ, and the number of viable bacteria in group Ⅳ is lesser than Group Ⅱ.
3. The number of viable bacteria of liver and spleen are gradually decreased according to time in group Ⅱ and Ⅳ and the number of viable bacteria in group Ⅳ is lesser than group Ⅱ in liver and spleen.
4. The microscopic finding of liver in group Ⅳ which show severe congestion, infiltration of PMN cells in sinusoid, increased kupffer cells and peripheral type necrosis of hepatic lobule.
5. The microscopic finding of spleen in group Ⅳ which show severe congestion, infiltration of PMN cells in all field and enlargen splenic corpuscle with marginal necorsis.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|